محیط کشت Fluid Thioglycolate Medium

Thioglycollate Medium                                            .                                                                                                   

MT – 3002

Thioglycollate medium is used for cultivation of anaerobic, micro-aerophilic and aerobic microorganisms and for sterility testing procedures.

Composition (gr /lit):

Tryptone ……………………..….15

Sodium chloride ……..…………2.5

Glucose …………..……….……5.5

Yeast extract …..……..…………. 5

L – Cystine………. ….……….…0.5

Sodium thioglycollate ………….0.5

Resazurin …………………….0.001

Agar …………..….……..……..0.75

pH : 7.1 ± 0.2 (at 25ᵒC)

Directions :

Suspend 29.8 gr/lit in distilled water. Heat to boiling to dissolve the medium completely.

Autoclave (15 min, at 15 lbs pressure, 121ᵒc).Store in a cool dark place preferably below 25ᵒC.

Thioglycollate Medium

Thioglycollate media is used for cultivation of anaerobic, micro-aerophilic and aerobic microorganisms and for sterility testing procedures. No paraffin or special seal is necessary, nor is a anaerobic jar required for the cultivation of anaerobes.The reducing components Thioglycollate and Cystine ensure adequate anaerobiosis even in the case of fastidious anaerobes.It is well buffered so that acide or alkalin inocula produce negligible alteration in the reaction of the medium.This culture medium is used for the cultivation of Clostridium species and also used  for the Blood culture Media.

Cultuer media within 24 – 48 hours incubation at 35 – 37ᵒc.

Storange and shelf-life :

Store dry and keep tightly closed,store at 15-25ᵒc protect from light and the prepared medium at 2 – 8ᵒc.

Quality Control :

Appearance of Powder: Yellowish coloured, homogeneous free flowing powder.

Gelling: Semi solid,with 0.75% agar.

Colour and Clarity: Yellowish coloured, clear to very slightly opalescent fluid with upper 10% or less medium pink on standing.

Organisms                                    Growth

1                                                  +

2                                                  +

3                                                  +

4                                                  +

1-Clostridium sporogenes ATCC 11437

2-Bacillus subtilis  ATCC 6633

3-Staphylococcus aureus ATCC 25923

4-Streptococcus pyogenes ATCC 19615